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dylight 488 antibody kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories dylight 488 antibody kit
    Dylight 488 Antibody Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dylight+488+anti+mouse/VectaFluor+Excel+Amplified+DyLight+488+Anti-Mouse+IgG+Kit/pmc13102498-133-15-20
    Average 93 stars, based on 44 article reviews
    dylight 488 antibody kit - by Bioz Stars, 2026-10
    93/100 stars

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    Incubation:

    Article Title: Skeletal Muscle Microvascular Changes in Response to Short-Term Blood Flow Restricted Training—Exercise-Induced Adaptations and Signs of Perivascular Stress
    Article Snippet: In brief, transverse cut serial sections (8 μm) were fixed (4% formaldehyde, Triton-X buffer) and subsequently blocked (X0909, Dako, Glostrup, Denmark) for 10 min. .. Sections were incubated for 60 min in room temperature with primary antibodies: CD31 (1:100, M0823, Dako) and neural/glial antigen 2 (NG2, 1:200, B5320, Merck Millipore, Darmstadt, Germany), while subsequently incubated with secondary antibodies: DyLight-488 anti-mouse (Vector, DK-2488, Burlingame, CA, United States) and biotinylated anti-rabbit (1:200; Vector) followed by DyLight-549 Streptavidin (1:500, Vector). .. A second analysis was performed to visualize the basal lamina using laminin (Z0097, Dako; 1:1,000) and Alexa-488 goat-anti-mouse (A11029, TS; 1:1,000).

    Article Title: TLR7 Expression Is Associated with M2 Macrophage Subset in Calcific Aortic Valve Stenosis
    Article Snippet: Next, sections were incubated overnight at 4 °C with primary antibodies against CD163 (pre-diluted, Abcam, Cambridge, UK), CD68 (1:50, Dako, Glostrup, Denmark), CD3 (pre-diluted, Biocare Medical, Pacheco, CA, USA ), and CD206 (1:50, R&D Systems, Minneapolis, USA) followed by an overnight incubation at 4 °C with TLR7 antibody (1:100, Novus Biologicals, Cambridge, UK). .. Sections were incubated with the following secondary antibodies: Dylight 488 anti-mouse and Dylight 594 anti-rabbit (1:300) (Vector Laboratories, Burlingame, CA). ..

    Staining:

    Article Title: Actl7b -deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis
    Article Snippet: .. Sections stained with anti-DYNLL1, anti-DYNLL2 and anti-ACTIN were processed using the VectaFluorTM Horse Anti-Rabbit IgG, DyLight® 488 Antibody Kit (Vector Laboratories, Burlingame, CA, USA; DI-1788), sections stained against Ezrin were processed with the VectaFluorTM Anti-Mouse IgG, DyLight® 594 Kit (Vector Laboratories, DI-2794), sections stained against PRM2 and ODF2 were processed with the VectaFluorTM Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit, DyLight® 488 Anti-Mouse (Vector Laboratories; DK-8828) and sections stained with anti-ACTL7B were processed using the Vectastain® ABC-AP Kit (Vector Laboratories; AK-5001) and ImmPACT® Vector® Red alkaline phosphatase substrate (Vector Laboratories; SK-5105). ..

    Immunofluorescence:

    Article Title: Actl7b -deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis
    Article Snippet: .. Sections stained with anti-DYNLL1, anti-DYNLL2 and anti-ACTIN were processed using the VectaFluorTM Horse Anti-Rabbit IgG, DyLight® 488 Antibody Kit (Vector Laboratories, Burlingame, CA, USA; DI-1788), sections stained against Ezrin were processed with the VectaFluorTM Anti-Mouse IgG, DyLight® 594 Kit (Vector Laboratories, DI-2794), sections stained against PRM2 and ODF2 were processed with the VectaFluorTM Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit, DyLight® 488 Anti-Mouse (Vector Laboratories; DK-8828) and sections stained with anti-ACTL7B were processed using the Vectastain® ABC-AP Kit (Vector Laboratories; AK-5001) and ImmPACT® Vector® Red alkaline phosphatase substrate (Vector Laboratories; SK-5105). ..

    Article Title: Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice
    Article Snippet: .. Slides were then double-stained with fluorescent secondary antibodies using the VectaFluorTM Duet Immunofluorescence Double Labeling Kit, DyLight ® 594 Anti-Rabbit (red), DyLight ® 488 Anti-Mouse (green) (Vectorlabs, Burlingame, USA), DAPI counterstained and coverslipped with ProLongTM Gold antifade reagent with DAPI (Thermo Fisher Scientific, Waltham, USA). ..

    Article Title: Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice
    Article Snippet: .. Slides were then double-stained with fluorescent secondary antibodies using the VectaFluor Duet Immunofluorescence Double Labeling Kit, DyLight 594 Anti-Rabbit (red), DyLight 488 Anti-Mouse (green) (Vectorlabs, Burlingame, USA), DAPI counterstained and coverslipped with ProLong Gold antifade reagent with DAPI (Thermo Fisher Scientific, Waltham, USA). ..

    Labeling:

    Article Title: Actl7b -deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis
    Article Snippet: .. Sections stained with anti-DYNLL1, anti-DYNLL2 and anti-ACTIN were processed using the VectaFluorTM Horse Anti-Rabbit IgG, DyLight® 488 Antibody Kit (Vector Laboratories, Burlingame, CA, USA; DI-1788), sections stained against Ezrin were processed with the VectaFluorTM Anti-Mouse IgG, DyLight® 594 Kit (Vector Laboratories, DI-2794), sections stained against PRM2 and ODF2 were processed with the VectaFluorTM Duet Immunofluorescence Double Labeling Kit, DyLight® 594 Anti-Rabbit, DyLight® 488 Anti-Mouse (Vector Laboratories; DK-8828) and sections stained with anti-ACTL7B were processed using the Vectastain® ABC-AP Kit (Vector Laboratories; AK-5001) and ImmPACT® Vector® Red alkaline phosphatase substrate (Vector Laboratories; SK-5105). ..

    Article Title: Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice
    Article Snippet: .. Slides were then double-stained with fluorescent secondary antibodies using the VectaFluorTM Duet Immunofluorescence Double Labeling Kit, DyLight ® 594 Anti-Rabbit (red), DyLight ® 488 Anti-Mouse (green) (Vectorlabs, Burlingame, USA), DAPI counterstained and coverslipped with ProLongTM Gold antifade reagent with DAPI (Thermo Fisher Scientific, Waltham, USA). ..

    Article Title: Loss of the cleaved-protamine 2 domain leads to incomplete histone-to-protamine exchange and infertility in mice
    Article Snippet: .. Slides were then double-stained with fluorescent secondary antibodies using the VectaFluor Duet Immunofluorescence Double Labeling Kit, DyLight 594 Anti-Rabbit (red), DyLight 488 Anti-Mouse (green) (Vectorlabs, Burlingame, USA), DAPI counterstained and coverslipped with ProLong Gold antifade reagent with DAPI (Thermo Fisher Scientific, Waltham, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The effects of losartan on cytomegalovirus infection in human trabecular meshwork cells
    Article Snippet: Recombinant human TGF-β1 from R&D Systems (Minneapolis, MN, USA), losartan from Selleck Chemicals (Houston, TX, USA), dexamethasone from Sigma-Aldrich (St. Louis, MO, USA), ganciclovir from Selleck Chemicals, normal horse serum (RTU Vectastain Universal Elite ABC Kit) from Vector Laboratories (Burlingame, CA, USA), murine monoclonal anti-CMV IE antibody (anti-CMV Immediate Early Antigen Antibody, #LS-C103255) from LSBio (Seattle, WA, USA), a goat anti-mouse antibody (VectaFluor R.T.U. .. DyLight 488 anti-mouse) from Vector Laboratories, rhodamine phalloidin and Vecta-Stain mounting media (VECTASHIELD) from Invitrogen (Carlsbad, CA, USA), a TGF-β1 enzyme-linked immunosorbent assay (ELISA) kit (Total TGF-β1 ELISA Kit with precoated plates) from BioLegend (San Diego, CA, USA), and a MTS assay kit (CellTiter 96 ® AQueous One Solution Cell Proliferation Assay) from Promega (Medison, WI, USA). ..

    MTS Assay:

    Article Title: The effects of losartan on cytomegalovirus infection in human trabecular meshwork cells
    Article Snippet: Recombinant human TGF-β1 from R&D Systems (Minneapolis, MN, USA), losartan from Selleck Chemicals (Houston, TX, USA), dexamethasone from Sigma-Aldrich (St. Louis, MO, USA), ganciclovir from Selleck Chemicals, normal horse serum (RTU Vectastain Universal Elite ABC Kit) from Vector Laboratories (Burlingame, CA, USA), murine monoclonal anti-CMV IE antibody (anti-CMV Immediate Early Antigen Antibody, #LS-C103255) from LSBio (Seattle, WA, USA), a goat anti-mouse antibody (VectaFluor R.T.U. .. DyLight 488 anti-mouse) from Vector Laboratories, rhodamine phalloidin and Vecta-Stain mounting media (VECTASHIELD) from Invitrogen (Carlsbad, CA, USA), a TGF-β1 enzyme-linked immunosorbent assay (ELISA) kit (Total TGF-β1 ELISA Kit with precoated plates) from BioLegend (San Diego, CA, USA), and a MTS assay kit (CellTiter 96 ® AQueous One Solution Cell Proliferation Assay) from Promega (Medison, WI, USA). ..

    Proliferation Assay:

    Article Title: The effects of losartan on cytomegalovirus infection in human trabecular meshwork cells
    Article Snippet: Recombinant human TGF-β1 from R&D Systems (Minneapolis, MN, USA), losartan from Selleck Chemicals (Houston, TX, USA), dexamethasone from Sigma-Aldrich (St. Louis, MO, USA), ganciclovir from Selleck Chemicals, normal horse serum (RTU Vectastain Universal Elite ABC Kit) from Vector Laboratories (Burlingame, CA, USA), murine monoclonal anti-CMV IE antibody (anti-CMV Immediate Early Antigen Antibody, #LS-C103255) from LSBio (Seattle, WA, USA), a goat anti-mouse antibody (VectaFluor R.T.U. .. DyLight 488 anti-mouse) from Vector Laboratories, rhodamine phalloidin and Vecta-Stain mounting media (VECTASHIELD) from Invitrogen (Carlsbad, CA, USA), a TGF-β1 enzyme-linked immunosorbent assay (ELISA) kit (Total TGF-β1 ELISA Kit with precoated plates) from BioLegend (San Diego, CA, USA), and a MTS assay kit (CellTiter 96 ® AQueous One Solution Cell Proliferation Assay) from Promega (Medison, WI, USA). ..



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